Journal: Natural Products and Bioprospecting

Article Title: Diversity of oligosaccharides in lipooligosaccharides of Akkermansia muciniphila and its anti-atherosclerotic activity

doi: 10.1007/s13659-026-00612-4

Figure Lengend Snippet: ALOS ameliorates gut barrier function and promotes IL-22 production. A C-reactive protein (hs-CRP). B LPS. C Tumour necrosis factor-alpha (TNF-α). D Interleukin-6 (IL-6). E Interleukin 1β (IL-1β). F Interleukin 10 (IL-10). G Representative AB-PAS staining of ileum pathological sections (scale bar, 100 μm, n = 4). H Plasma IL-23. I Plasma IL-22. J Representative immunofluorescent staining of IL-22 in the ileum. n = 4 mice for each group. Scale bar, 20 μm. K Western blotting of the IL-22 and β-actin levels in the ileum tissue of ApoE −/− mice. *P < 0.05; **P < 0.01. One-way ANOVA (with Tukey's HSD) test in A–I. Unpaired t-test in J–K.

Article Snippet: The interleukin-6 (IL-6) (SEKM-0007; Solarbio), tumor necrosis factor-alpha (TNF-α) (RK00027; Abclonal), interleukin-1β (IL-1β) (MM-0040M1; MEIMIAN), interleukin-10 (IL-10) (1418033–25-6; Solarbio), interleukin-22 (IL-22) (E-MSEL-M0040; Elabscience BiotechnologyCo., Ltd.), interleukin-23 (IL-23) (RK00017; Abclonal), oxidized LDL (ox-LDL), very low density lipoprotein cholesterol (VLDL) (ml037709-2; Mlbio), and lipopolysaccharide (LPS) (WLCSJZF202535; AMOY LUNCHANGSHUO) levels in the plasma and ileum were determined in commercial ELISA kits.

Techniques: Staining, Clinical Proteomics, Western Blot

Journal: Journal of Nanobiotechnology

Article Title: Mitochondria-targeted nanozyme system for psoriasis treatment

doi: 10.1186/s12951-026-04068-z

Figure Lengend Snippet: Therapeutic effects of CMH@lip@Res-TCeO2 on IMQ-induced psoriasiform skin inflammation. ( A - B ) Macroscopic observation of dorsal skin lesions in mice, evaluating erythema, scaling, and inflammatory infiltration; ( C ) H&E staining to assess histopathological features and changes in epidermal thickness (scale bar: 50 μm); ( D ) Ly6G IF staining to evaluate neutrophil infiltration in skin tissues (scale bar: 50 μm); ( E ) Colorimetric assay to measure MPO activity in skin tissues; ( F - G ) Detection of ROS and MDA levels using fluorescent probes and evaluation of CAT, SOD, and GSH activities using colorimetric assays; ( H ) IHC analysis of Ki67 expression as a proliferation marker in skin tissues (scale bar: 50 μm); ( I ) ELISA quantification of IL-23, IL-1β, IL-17 A, and TNF-α levels in skin homogenates. Each group included six animals. *p < 0.05, **p < 0.01, ***p < 0.001

Article Snippet: Cytokine levels in cell culture supernatants and tissue homogenates were measured using ELISA kits for IL-1β (DY401), IL-6 (M6000B), TNF-α (MTA00B), IL-17 A (DY5390), and IL-23 (M2300) (R&D Systems, USA).

Techniques: Staining, Colorimetric Assay, Activity Assay, Expressing, Marker, Enzyme-linked Immunosorbent Assay

Journal: bioRxiv

Article Title: Spatiotemporal Atlas of Pro-Inflammatory (NF-κB) and Anti-Inflammatory (STAT6) Signalling Using Reporter Mice during mRNA Vaccination

doi: 10.64898/2026.01.29.702227

Figure Lengend Snippet: Bone marrow–derived dendritic cells were treated with 2.5 µg of modified or unmodified mRNA encapsulated in LNPs, with an equivalent amount of empty LNPs, or with vehicle (water). Representative histograms show the percentage of CD40-CD80-CD86-CCR7, and MHCII positive cells assessed by flow cytometry, and IL-12 concentrations measured by ELISA ± SEM at 24 or 48 h post treatment (n = 9 at 24 h; n = 3 at 48 h). ***p < 0.001 versus vehicle (two-way ANOVA, Dunnett’s test).

Article Snippet: The concentration of IL-12 in supernatants from BM-DCs was determined by ELISA using the Mouse IL-12/IL-23 p40 Allele-specific DuoSet ELISA kit (R&D Systems, Cat. DY499) following the manufacturer’s instructions.

Techniques: Derivative Assay, Modification, Flow Cytometry, Enzyme-linked Immunosorbent Assay

Journal: bioRxiv

Article Title: Spatiotemporal Atlas of Pro-Inflammatory (NF-κB) and Anti-Inflammatory (STAT6) Signalling Using Reporter Mice during mRNA Vaccination

doi: 10.64898/2026.01.29.702227

Figure Lengend Snippet: (A, C) Representative ex vivo bioluminescence images of organs dissected from NFκB -luc2 and STAT6 -luc2 reporter mice at (A) 5 hours or (C) 29 days after treatment. Signals are shown using pseudocolors according to the scale bar. Quantification of organ bioluminescence is shown in the bar graphs, representing mean photon emission (photons/s/cm²/sr) ± SEM (n = 6). *p < 0.05, **p < 0.01, ** p < 0.001 versus LNP (two-way ANOVA, Dunnett’s test).Thym: thymus, LN: inguinal lymph nodes, Dued.: duodenum. (B, D) Cytokine levels in plasma were measured (B) 5 hours after administration or (D) at day 29. Cytokine concentrations are presented as bar graphs ± SEM (n = 3). *p < 0.05, * p < 0.01 versus LNP (one-way ANOVA, Dunnett’s test). (E) Antigen-specific antibodies were measured in plasma at day 29 by ELISA. Bar graphs represent mean absorbance ± SEM (n = 12). ** p < 0.001 versus LNP (two-way ANOVA, Dunnett’s test).

Article Snippet: The concentration of IL-12 in supernatants from BM-DCs was determined by ELISA using the Mouse IL-12/IL-23 p40 Allele-specific DuoSet ELISA kit (R&D Systems, Cat. DY499) following the manufacturer’s instructions.

Techniques: Ex Vivo, Clinical Proteomics, Enzyme-linked Immunosorbent Assay